Recombinant Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Gln20-Glu351.
Biotinylated
Yes
产物规格
靶点
GFRAL/GFR alpha-like
份子又名
Accession
种属
Cynomolgus
卵白序列
Gln20-Glu351
份子量
The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
活性
Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 76.3ng/ml determined by ELISA. See testing image for detail.
产物标签
N-His-Avi
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Liquid
运输前提
Shipped with blue ice.
制剂
Supplied as 0.22um filtered solution in 20mM PB, 400mM NaCl (pH 6.0).
复溶
存储和不变性
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.