Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gln18-Ser740.
Biotinylated
Yes
产物规格
靶点
ACE2/ACEH
份子又名
ACE-2; ACEH; ACE2
Accession
种属
Human
卵白序列
Gln18-Ser740
份子量
The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result.
活性
Immobilized SARS-COV-2 S1, His Tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human ACE2, His Tag with EC50 of 0.24ug/ml determined by ELISA. See testing image for detail.
产物标签
C-His-Avi
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.