Recombinant Biotinylated Human Mature TGF beta 3 Protein is expressed from HEK293 with Avi tag at the C-terminal.It contains Ala301-Ser412.
Biotinylated
Yes
产物规格
靶点
Mature TGF beta 3
份子又名
Accession
种属
Human
卵白序列
Ala301-Ser412
份子量
The protein has a predicted MW of 14.53 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human TGF-beta RII, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 3, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
产物标签
C-Avi
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.