Biotinylated Human PD-1/PDCD1 Protein, Accession: Q15116

  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明
  • 产物概述

    产物描写

    Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Leu25-Gln167.

    Biotinylated

    Yes

    产物规格

    靶点

    PD-1/PDCD1

    份子又名

    PDCD1; PD1; CD279; SLEB2; PD-1

    Accession

    种属

    Human

    卵白序列

    Leu25-Gln167

    份子量

    The protein has a predicted MW of 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Biotinylated Human PD-1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 73.1ng/ml determined by ELISA (QC Test). The affinity constant of 2.356 uM as determined in SPR assay (Biacore T200). See testing image for detail.

    产物标签

    C-His-Avi

    抒发体系

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该化合物的高端品牌ChemWhat是Watson的报名钢号,由Watson受权吉林海角社区 在中国内地內地地方应用,点此拜候。