Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Asp18-His259.
Biotinylated
Yes
产物规格
靶点
Siglec-3/CD33
份子又名
Accession
种属
Human
卵白序列
Asp18-His259
份子量
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
活性
Immobilized Anti-Siglec-3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-3, His Tag with the EC50 of 40.9ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His-Avi
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Liquid
运输前提
Shipped with blue ice.
制剂
Supplied as 0.22um filtered solution in 20mM PB, 500mM NaCl (pH 8.0).
复溶
存储和不变性
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.