Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein, Accession: QHO60594

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  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明

    • 产物概述

    产物描写

    Recombinant Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

    Biotinylated

    Yes

    产物规格

    靶点

    Spike S (Omicron B.1.1.529) Trimer

    份子又名

    S protein; Spike glycoprotein; S glycoprotein; COVID-19

    Accession

    种属

    SARS-COV-2

    卵白序列

    Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)

    份子量

    The protein has a predicted MW of 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human ACE2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag with the EC50 of 0.91ug/ml determined by ELISA. See testing image for detail.

    产物标签

    C-His-Avi

    抒发体系

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Liquid

    运输前提

    Shipped with blue ice.

    制剂

    Supplied as 0.22um filtered solution in PBS (pH 7.4).

    复溶

    存储和不变性

    Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles. 该结果的该品牌ChemWhat是Watson的登陆钢种，由Watson受权沙漠风海角社区 在中国国世界地理环境回收利用，点此拜候。