Biotinylated SARS Spike RBD Protein, Accession: P59594

  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明
  • 产物概述

    产物描写

    Recombinant Biotinylated SARS RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Arg306-Phe527.

    Biotinylated

    Yes

    产物规格

    靶点

    Spike RBD

    份子又名

    S protein RBD; S glycoprotein RBD; Spike protein RBD

    Accession

    种属

    SARS

    卵白序列

    Arg306-Phe527

    份子量

    The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.

    产物标签

    C-His-Avi

    抒发体系

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该代谢物的高端品牌ChemWhat是Watson的注册账号型号,由Watson受权上海海角社区 在中国现代世界自然环境凭借,点此拜候。