Recombinant Biotinylated SARS RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Arg306-Phe527.
Biotinylated
Yes
产物规格
靶点
Spike RBD
份子又名
S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession
种属
SARS
卵白序列
Arg306-Phe527
份子量
The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
产物标签
C-His-Avi
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.