Recombinant Cynomolgus Alkaline Phosphatase Protein (Placental type) is expressed from HEK293 with His tag at the C-terminal.It contains Ile21-Thr503.
Biotinylated
产物规格
靶点
Alkaline Phosphatase
份子又名
Accession
种属
Cynomolgus
卵白序列
Ile21-Thr503
份子量
The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Tris-Bis PAGE result.
活性
The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.