Recombinant Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ser17-Glu460.
Biotinylated
产物规格
靶点
ANGPTL3
份子又名
Accession
种属
Cynomolgus
卵白序列
Ser17-Glu460
份子量
The protein has a predicted MW of 52.86 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
活性
Immobilized Cynomolgus ANGPTL3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.