Recombinant Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Gln20-Glu351.
Biotinylated
产物规格
靶点
GFRAL/GFR alpha-like
份子又名
GFR alpha-like; GFRAL; GRAL; C6orf144
Accession
种属
Cynomolgus
卵白序列
Gln20-Glu351
份子量
The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
活性
Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA. See testing image for detail.
产物标签
N-His-Avi
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in 20mM PB, 0.4M NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM PB, 0.4M NaCl (pH 6.0).
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.