Recombinant Cynomolgus HGF R/c-MET Protein is expressed from HEK293 with His tag at the C-terminal.It contains Glu25-Asn930.
Biotinylated
产物规格
靶点
HGF R/c-MET
份子又名
Accession
种属
Cynomolgus
卵白序列
Glu25-Asn930
份子量
The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 69.9 kDa (β chain). Due to glycosylation, the protein migrates to 45-52 kDa (α chain) and 75-82 kDa (β chain) based on Tris-Bis PAGE result.
活性
Immobilized Cynomolgus HGF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 38.4ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.