Recombinant Cynomolgus Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag at the C-terminal.It contains Phe31-Pro993(ITGAV) acidic tail & Gly27-Asp718(ITGB3) basic tail.
The protein has a predicted MW of 112.31 kDa (ITGAV) & 81.27 kDa (ITGB3). Due to glycosylation, the protein migrates 95-140 to kDa based on Tris-Bis PAGE result.
活性
Immobilized Cynomolgus ITGAV&ITGB3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-alpha V beta 3 Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Liquid
运输前提
Shipped with blue ice.
制剂
Supplied as 0.22um filtered solution in PBS (pH 7.4).
复溶
存储和不变性
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.