Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M).
Biotinylated
产物规格
靶点
FcRn
份子又名
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Accession
Q8SPV9(FCGRT)&Q8SPW0(B2M)
种属
Cynomolgus/Rhesus macaque
卵白序列
Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
份子量
The protein has a predicted MW of 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result.
活性
Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 uM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.