Recombinant Human APLN Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Gly23-Phe77.
Biotinylated
产物规格
靶点
APLN
份子又名
Apelin;APJ endogenous ligand;APEL
Accession
种属
Human
卵白序列
Gly23-Phe77
份子量
The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human APLN, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 22.2ng/ml determined by ELISA. See testing image for detail.
产物标签
C-hFc
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.