The protein has a predicted MW of 22.7 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human BDCA-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. See testing image for detail.
产物标签
N-His-Avi
抒发体系
Expi293
纯度
> 95% as determined by Tris-Bis PAGE
内毒素
Less than 1EU per μg by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 200mM L-Arginine (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.