The protein has a predicted MW of 21 kDa. Due to the protein can cleaved into two chains, so it migrates to 15-18 kDa and 22-25 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). See testing image for detail.
产物标签
N-His,SUMO
抒发体系
E.coli
纯度
> 95% as determined by Tris-Bis PAGE
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in 20mM Tris,0.3M NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.