The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 49.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.958 uM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
C-hFc
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.