The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human CD30, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc (IgG2a) Tag with the EC50 of 31.9ng/ml determined by ELISA. See testing image for detail.
产物标签
N-mFc
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC;
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.