Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein, Accession: P07766(CD3E)&P04234(CD3D)

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  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明

    • 产物概述

    产物描写

    Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with His tag at the C-terminal.It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3D).

    Biotinylated

    产物规格

    靶点

    CD3E&CD3D/CD3 epsilon&CD3 delta

    份子又名

    CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon

    Accession

    P07766(CD3E)&P04234(CD3D)

    种属

    Human

    卵白序列

    Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)

    份子量

    The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human CD3E&CD3D at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA (QC Test). The affinity constant of 0.36nM as determined in SPR assay (Biacore T200). See testing image for detail.

    产物标签

    C-His

    抒发体系

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该物品的国产品牌ChemWhat是Watson的祖册品牌，由Watson受权吉林海角社区 在国内 地区温带季风气候使用，.