Human CD47 Protein, Accession: Q08722

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  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明

    • 产物概述

    产物描写

    Recombinant Human CD47 is expressed from HEK293 with His tag at the C-terminal.It contains Gln19-Pro139.

    Biotinylated

    产物规格

    靶点

    CD47

    份子又名

    CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6

    Accession

    种属

    Human

    卵白序列

    Gln19-Pro139

    份子量

    The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA (QC Test). The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.

    产物标签

    C-His

    抒发体系

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该货物的产品ChemWhat是Watson的注册会员品种，由Watson受权上海海角社区 在我国的大陆台湾区域合理利用，点此拜候。