Human CD73/NT5E Protein, Accession: P21589

  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明
  • 产物概述

    产物描写

    Recombinant Human CD73/NT5E Protein is expressed from HEK293 with hFC tag at the C-terminal.It contains Trp27-Lys547.

    Biotinylated

    产物规格

    靶点

    CD73/NT5E

    份子又名

    CD73; NT5E; NTE; 5′ nucleotidase (CD73); 5′-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55′-nucleotidase; NT5; NTE; CALJA

    Accession

    种属

    Human

    卵白序列

    Trp27-Lys547

    份子量

    The protein has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human CD73, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test). See testing image for detail.

    产物标签

    C-hFc

    抒发体系

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该化合物的厂家ChemWhat是Watson的注册账号类型,由Watson受权山东海角社区 在国家中国地区根据,点此拜候。