Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.
Biotinylated
产物规格
靶点
EGF
份子又名
HOMG4; EGF; URG
Accession
种属
Human
卵白序列
Asn971-Arg1023
份子量
The protein has a predicted MW of 5.6 kDa same as Tris-Bis PAGE result.
活性
Immobilized Human EGF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.3ug/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
No Tag
抒发体系
E.coli
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.