Human GDF15 Protein, Accession: Q99988

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  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明

    • 产物概述

    产物描写

    Recombinant Human GDF15 Protein is expressed from Expi293 with mFc (IgG2a) tag at the N-terminal.It contains Ala197-Ile308.

    Biotinylated

    产物规格

    靶点

    GDF15

    份子又名

    Accession

    种属

    Human

    卵白序列

    Ala197-Ile308

    份子量

    The protein has a predicted MW of 37.46 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human GFRAL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GDF15, mouse IgG2a Fc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.

    产物标签

    N-mFc

    抒发体系

    Expi293

    纯度

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    内毒素

    Less than 1EU per μg by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该物质的的品牌ChemWhat是Watson的注册账号钢材型号，由Watson受权广东海角社区 在中大陸地域性借助，点此拜候。