Human GDF15 Protein, Accession: Q99988

  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明
  • 产物概述

    产物描写

    Recombinant Human GDF15 Protein is expressed from E.coli with His tag at the N-terminal.It contains Ala197-Ile308.

    Biotinylated

    产物规格

    靶点

    GDF15

    份子又名

    GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

    Accession

    种属

    Human

    卵白序列

    Ala197-Ile308

    份子量

    The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human GDF15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 22.8ng/ml determined by ELISA. The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail.

    产物标签

    N-His

    抒发体系

    E.coli

    纯度

    > 95% as determined by Tris-Bis PAGE

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该代谢物的高端品牌ChemWhat是Watson的注册网站钢种,由Watson受权深圳海角社区 在中国国中国香港具体巧用,点此拜候。