Recombinant Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Ile23-Ser328 (IL-12B) & Arg23-Ser219 (IL-12A).
The protein has a predicted MW of 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His-Avi
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.