Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162.
Biotinylated
产物规格
靶点
IL-15
份子又名
L15; IL-15; IL-15MGC9721; Interleukin-15
Accession
种属
Human
卵白序列
Asn49-Ser162
份子量
The protein has a predicted MW of 12.8 kDa same as Tris-Bis PAGE result.
活性
Immobilized Human IL-15 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 22.1ng/ml determined by ELISA. The affinity constant of 5.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
No Tag
抒发体系
E.coli
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.