The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.7 determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
C-hFc
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.