Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
Biotinylated
产物规格
靶点
IL-7
份子又名
IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
Accession
种属
Human
卵白序列
Asp26-His177
份子量
The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result.
活性
Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml determined by ELISA. The affinity constant of 22.66 nM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
No Tag
抒发体系
E.coli
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 25mM PB, 250mM NaCl (pH 6.8).
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.