Human KRAS G12D (HLA-A*11:01) Tetramer Protein, Accession: AAV53343

  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明
  • 产物概述

    产物描写

    Recombinant Human KRAS G12D(HLA-A*11:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M) and VVVGADGVGK peptide.

    Biotinylated

    产物规格

    靶点

    KRAS G12D (HLA-A*11:01) Tetramer

    份子又名

    MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD

    Accession

    种属

    Human

    卵白序列

    Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide

    份子量

    The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

    活性

    Immobilized KRAS G12D (HLA-A*11:01) (VVVGADGVGK) TCR at 1ug/ml (100ul/well) on the plate. Dose response curve for Human KRAS G12D (HLA-A*11:01) Tetramer, His Tag with the EC50 of 49.9ng/ml determined by ELISA. See testing image for detail.

    产物标签

    C-His-Avi

    抒发体系

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该货物的公司ChemWhat是Watson的注冊品牌,由Watson受权四川海角社区 在中国大内地省份合理利用,点此拜候。