The protein has a predicted MW of 13.2 kDa (monomer). Due to glycosylation, the protein migrates to 14-15 kDa (monomer) under reduced (R) condition and 26-30 kDa (dimer) under Non reducing (N) condition based on Tris-Bis PAGE result.
活性
Immobilized Human Mature TGF beta 1, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA (QC Test). The affinity constant of 0.35 uM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
No Tag
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine 150mM NaCl (pH 2.5).
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.