Human MMP-8 Protein, Accession: P22894

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  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明

    • 产物概述

    产物描写

    Recombinant Human MMP-8 Protein is expressed from HEK293 with His tag at the C-terminal. The protein needs to be activated by APMA to have hydrolytic activity.It contains Phe21-Gly467.

    Biotinylated

    产物规格

    靶点

    MMP-8

    份子又名

    MMP-8; PMNL-CL; CLG1; MMP8; Collagenase 2; PMNL-CL; HNC

    Accession

    种属

    Human

    卵白序列

    Phe21-Gly467

    份子量

    The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 3.5ug/ml determined by ELISA (QC Test). The affinity constant of 1.14 uM as determined in SPR assay (Biacore T200). See testing image for detail.

    产物标签

    C-His

    抒发体系

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in 50mM Tris,10mM CaCl2, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该货物的加盟品牌ChemWhat是Watson的注册成功钢材牌号，由Watson受权河北海角社区 在我国的内地省份再生利用，点此拜候。