Recombinant Human NY-ESO-1 (HLA-A*02:01) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide.
Biotinylated
产物规格
靶点
NY-ESO-1 (HLA-A*02:01) Tetramer
份子又名
Accession
种属
Human
卵白序列
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide
份子量
The protein has a predicted MW of 258 kDa. The protein migrates to 160-250 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on Tris-Bis PAGE result under Non reducing (N) condition.
活性
Immobilized Human NY-ESO-1 (HLA-A*02:01) Tetramer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His-Avi
抒发体系
E.coli
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per μg by the LAL method.
运输及存储
产物表面
Liquid
运输前提
Shipped with blue ice.
制剂
Supplied as 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
复溶
存储和不变性
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.