Recombinant Human PCSK9 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gln31-Gln692.
Biotinylated
产物规格
靶点
PCSK9
份子又名
PC9; PCSK9; FH3; HCHOLA3; LDLCQ1; NARC-1
Accession
种属
Human
卵白序列
Gln31-Gln692
份子量
Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result.
活性
Immobilized Human PCSK9, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). The affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Liquid
运输前提
Shipped with blue ice.
制剂
Supplied as 0.22um filtered solution in 20mM PB, 300mM NaCl, 10% Glycerol (pH 7.4).
复溶
存储和不变性
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.