Recombinant Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gly20-Pro516.
Biotinylated
产物规格
靶点
Siglec-4a/MAG
份子又名
MAG; Siglec4a; Siglec-4a; S-MAG; GMA
Accession
种属
Human
卵白序列
Gly20-Pro516
份子量
The protein has a predicted MW of 55.8 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human Siglec-4a, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.