Human TGF-alpha Protein, Accession: P01135

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  • 产物概述
  • 产物规格
  • 运输及存储
  • 品牌申明

    • 产物概述

    产物描写

    Recombinant Human TGF-alpha Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Val40-Ala89.

    Biotinylated

    产物规格

    靶点

    TGF-alpha

    份子又名

    ETGF; TGF-alpha; TGF type 1; TGFA

    Accession

    种属

    Human

    卵白序列

    Val40-Ala89

    份子量

    The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.

    产物标签

    N-hFc

    抒发体系

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产物表面

    Lyophilized

    运输前提

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和不变性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 该生成物的品牌标志ChemWhat是Watson的注测钢号，由Watson受权四川海角社区 在国内国内地区采用，点此拜候。