Recombinant Human TNFSF15 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-terminal.It contains Asp91-Leu251.
Biotinylated
产物规格
靶点
TNFSF15 trimer
份子又名
TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
Accession
种属
Human
卵白序列
Asp91-Leu251
份子量
The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human TNFSF15 Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. The affinity constant of 3.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
产物标签
N-His-Flag
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.