mRNA Cap 2′-O-Methyltransferase, utilizing S-adenosylmethionine (SAM) as a methyl group donor, adds a methyl group at the 2′-O position of the first nucleotide adjacent to the Cap0 structure at the 5′ end of RNA to obtain m7Gppp5’mN Cap1 structure. Cap1 structure is a signature of self RNA.and has been shown to modulate binding of innate immune sensors. For instance, the binding affinity of IFIT-1 for Cap1 is much weaker than for 5′ triphosphate or Cap0 structure; Cap1 structure abrogates RIG-1 signaling and prevents detection by MDA5.
NOTE: Vaccinia Capping Enzyme #GMP-VCS-VE101) is required to generate Cap0 structure.
This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038029.
Biotinylated
产物规格
靶点
份子又名
Accession
种属
卵白序列
份子量
活性
产物标签
抒发体系
纯度
内毒素
运输及存储
产物表面
Liquid
运输前提
Shipped with blue ice
制剂
复溶
存储和不变性
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
RNase III, Accession: