Recombinant Beta-lactamase TEM-1 (rTEM-1)
英文全名
Recombinant Beta-lactamase TEM-1 (rTEM-1)英文又名
卵白布局
氨基酸序列
MHPETLVKVK DAEDQLGARV GYIELDLNSG KILESFRPEE RFPMMSTFKV LLCGAVLSRV DAGQEQLGRR IHYSQNDLVE YSPVTEKHLT DGMTVRELCS AAITMSDNTA ANLLLTTIGG PKELTAFLHN MGDHVTRLDR WEPELNEAIP NDERDTTMPA AMATTLRKLL TGELLTLASR QQLIDWMEAD KVAGPLLRSA LPAGWFIADK SGAGERGSRG IIAALGPDGK PSRIVVIYTT GSQATMDERN RQIAEIGASL IKHWAccession
Gene ID
产物英文概述
Beta-lactamases are enzymes produced by some bacteria and are responsible for their resistance to beta-lactam antibiotics like penicillins, cephamycins, and carbapenems. The lactamase enzyme breaks the beta-lactam ring open and deactivates the molecule’s antibacterial properties because of a common element in these antibiotics molecular structure: a four-atom ring known as a beta-lactam. TEM-1 is the most commonly-encountered beta-lactamase in gram-negative bacteria. Up to 90 % of ampicillin resistance in E. coli is due to the production of TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H. influenzae and N. gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids residues.来历
份子量
生物活性
Fully biologically active when compared to standard. One unit of enzyme activity is defined as the amount of enzyme which will hydrolyze 1.0 umol of benzyl penicillin in presence of EDTA at pH 7.0 and at 25 °C.表面
Sterile Filtered White lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 100 mM Tris, pH 7.0.内毒素
消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Bush K, Jacoby GA, Medeiros AA. 1995. Antimicrob Agents Chemother. 39:1211-33.2. Ambler RP. 1980. Philos Trans R Soc Lond B Biol Sci. 289:321-31.3. Leinberger DM, Grimm V, Rubtsova M, et al. 2010. J Clin Microbiol. 48:460-71.纯度
> 95 % by SDS-PAGE.SDS-PAGE电泳
