Recombinant Bovine Platelet Factor-4/CXCL4 (rBoPF-4/CXCL4)
英文全名
Recombinant Bovine Platelet Factor-4/CXCL4 (rBoPF-4/CXCL4)英文又名
卵白布局
氨基酸序列
ESSFPATFVP LPADSEGGED EDLQCVCLKT TSGINPRHIS SLEVIGAGTH CPSPQLLATK KTGRKICLDQ QRPLYKKILK KLLDGDESAccession
Gene ID
产物英文概述
Platelet Factor-4 (PF-4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin. It interacts with a splice variant of the chemokine receptor CXCR3 besides be chemotactic for neutrophils, fibroblasts and monocytes. Recombinant bovine CXCL4 contains 88 amino acids. Specifically, Bovine and sheep CXCL4 share about an 85 % identity.来历
份子量
Approximately 9.5 kDa, a single non-glycosylated polypeptide chain containing 88 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.表面
Sterile Filtered White lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.内毒素
Less than 0.1 EU/ug of rBoPF-4/CXCL4 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
