Recombinant Hirudin (rHirudin)
英文全名
Recombinant Hirudin (rHirudin)英文又名
卵白布局
氨基酸序列
VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYLAccession
Gene ID
产物英文概述
来历
Pichia Pastoris份子量
Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues.生物活性
The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14,000 ATU/mg protein.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.内毒素
Less than 1 EU/ug of rHirudin as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Knapp A, Degenhardt T, Dodt J. 1992. J Biol Chem, 267: 24230-4.2. Pineo GFandHull RD. 1995. Curr Opin Hematol, 2: 380-5.3. Badimon L, Merino A, Badimon J, et al. 1991. Trends Cardiovasc Med, 1: 261-7.4. Nasir MA, Toth CA, Mittra RA. 1996. Am J Ophthalmol, 121: 554-60.5. Agnelli GandSonaglia F. 1997. Semin Thromb Hemost, 23: 143-8.纯度
> 96 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
