Recombinant Human Angiostatin (rHuAngiostatin)
英文全名
Recombinant Human Angiostatin (rHuAngiostatin)英文又名
卵白布局
氨基酸序列
VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLK GTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DGKRAPWCHT TNSQVRWEYC KIPSCDSSPAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 29.7 KDa, a single non-glycosylated polypeptide chain containing 259 amino acids.生物活性
Fully biologically active when compared to standard. The specific activity determined by an assay on anti-proliferation and anti-migration using endothelial cells in vitro and anti-angiogenesis in vivo is 5.5 × 105 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM NaAc, pH 5.5, 4 % mannitol.内毒素
Less than 1 EU/ug of rHuAngiostatin as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Takahashi S, Shinya T, Sugiyama A. 2010. J Pharmacol Sci, 112: 432-7.2. Frank KFandMuller-Ehmsen J. 2009. Heart, 95: 269-70.3. Perri SR, Annabi B, Galipeau J. 2007. FASEB J, 21: 3928-36.4. Kim KS, Kim HS, Park JS, et al. 2004. Cancer Gene Ther, 11: 441-9.5. Wahl ML, Owen CS, Grant DS. 2002. Endothelium, 9: 205-16.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
