Recombinant Human B-Cell Maturation Antigen/TNFRSF17 (rHuBCMA/TNFRSF17)
英文全名
Recombinant Human B-Cell Maturation Antigen/TNFRSF17 (rHuBCMA/TNFRSF17)英文又名
TNFRSF17, BCM, CD269卵白布局
氨基酸序列
AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNAAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 5.4 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg in the presence of 100 ng/ml human APRIL.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.内毒素
Less than 1 EU/ug of rHuBCMA as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Kim J, Gross JA, Dillon SR, et al. 2011. Autoimmunity, 44: 69-81.2. Sun J, Feng J, Li Y, et al. 2006. Biochem Biophys Res Commun, 346: 1158-62.3. Pelletier M, Thompson JS, Qian F, et al. 2003. J Biol Chem, 278: 33127-33.4. Laabi Y, Gras MP, Brouet JC, et al. 1994. Nucleic Acids Res, 22: 1147-54.纯度
> 98 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
