Recombinant Human Beta-defensin 3 (rHuBD-3)
英文全名
Recombinant Human Beta-defensin 3 (rHuBD-3)英文又名
Defensin beta3卵白布局
氨基酸序列
GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKKAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 5.2 kDa, a single non-glycosylated polypeptide chain containing 45 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 ug/ml, corresponding to a specific activity of > 33.3 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.内毒素
Less than 1 EU/ug of rHuBD-3 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Lioi AB, Reyes Rodriguez AL, Funderburg NT, et al. 2012. J Leukoc Biol, 92: 1083-91.2. Wang K, Wang JH, Baskaran H, et al. 2012. Head Neck Oncol, 4: 41.3. Lee JK, Park YJ, Kum KY, et al. 2012. Int Endod J,纯度
> 98 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
