Recombinant Human Brain Natriuretic Peptide (rHuBNP)
英文全名
Recombinant Human Brain Natriuretic Peptide (rHuBNP)英文又名
Brain natriuretic peptide 32, Gamma-brain natriuretic peptide, B-type Natriuretic Peptide, GC-B, BNP-32卵白布局
氨基酸序列
SPKMVQGSGC FGRKMDRISS SSGLGCKVLR RHAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 3.5 kDa, a single non-glycosylated polypeptide chain containing 32 amino acids.生物活性
No data available表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rHuBNP as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Matsuo H, Kangawa K, Minamino N. 1988. Tanpakushitsu Kakusan Koso, 33: 2438-50.2. Uehara Y, Shimizu H, Shimomura Y, et al. 1990. Neuropeptides, 17: 107-10.3. Hunt PJ, Espiner EA, Nicholls MG, et al. 1997. Peptides, 18: 1475-81.4. Richards AM, Lainchbury JG, Nicholls MG, et al. 2002. Trends Endocrinol Metab, 13: 151-5.5. Trojnarska O, Gwizdala A, Katarzynski S, et al. 2010. Int J Cardiol, 139: 241-7.纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
