Recombinant Human Ciliary Neurotrophic Factor (rHuCNTF)
英文全名
Recombinant Human Ciliary Neurotrophic Factor (rHuCNTF)英文又名
CNTF卵白布局
氨基酸序列
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKMAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rHuCNTF as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Lam A, Fuller F, Miller J, et al. 1991. Gene. 102:271-6.2. Bazan JF. 1991. Neuron. 7:197-208.3. McGregor NE, Poulton IJ, Walker EC, et al. 2010. Calcif Tissue Int. 86:261-70.4. Lambert PD, Anderson KD, Sleeman MW, et al. 2001. Proc Natl Acad Sci U S A. 98:4652-7.纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
