Recombinant Human Cu/Zn Superoxide Dismutase, His (rHuCu/ZnSOD,His)
英文全名
Recombinant Human Cu/Zn Superoxide Dismutase, His (rHuCu/ZnSOD,His)英文又名
SOD1卵白布局
氨基酸序列
MGHHHHHHHH HHSSGHIEGR HMTYARAAAR QARALEATKA VCVLKGDGPV QGIINFEQKE SNGPVKVWGS IKGLTEGLHG FHVHEFGDNT AGCTSAGPHF NPLSRKHGGP KDEERHVGDL GNVTADKDGV ADVSIEDSVI SLSGDHCIIG RTLVVHEKAD DLGKGGNEES TKTGNAGSRL ACGVIGIAQAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 39.9 kDa, a homodimer, non-glycosylated polypeptide chain containing 2 × 189 amino acids with Met, Gly and 10 × His at N-terminus.生物活性
Fully biologically active when compared to standard. The potency per mg was determined by Pyrogallic Acid method and was found to be more than 1.0 × 104 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rHuCu/Zn SOD, His as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Kwiatowski J, Skarecky D, Ayala FJ. 1992. Mol Phylogenet Evol, 1: 72-82.2. Bachus R, Claus A, Megow D, et al. 1995. J Neurol Sci, 129 Suppl: 93-5.3. Qi X, Guy J, Nick H, et al. 1997. Invest Ophthalmol Vis Sci, 38: 1203-12.4. Chou CM, Huang CJ, Shih CM, et al. 2005. Ann N Y Acad Sci, 1042: 303-13.5. Raja SB, Murali MR, Roopa K, et al. 2011. Biomed Pharmacother, 65: 560-8.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
