Recombinant Human Fibroblast Growth Factor 16 (rHuFGF-16)
英文全名
Recombinant Human Fibroblast Growth Factor 16 (rHuFGF-16)英文又名
卵白布局
氨基酸序列
AEVGGVFASL DWDLHGFSSS LGNVPLADSP GFLNERLGQI EGKLQRGSPT DFAHLKGILR RRQLYCRTGF HLEIFPNGTV HGTRHDHSRF GILEFISLAV GLISIRGVDS GLYLGMNERG ELYGSKKLTR ECVFREQFEE NWYNTYASTL YKHSDSERQY YVALNKDGSP REGYRTKRHQ KFTHFLPRPV DPSKLPSMSR DLFHYRAccession
Gene ID
产物英文概述
来历
份子量
Approximately 23.6 kDa, a single non-glycosylated polypeptide chain containing 206 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.表面
Sterile Colorless liquid.配方
Supplied as a 0.2 um filtered solution in 20 mM Tris-HCl, 1 M NaCl, pH 9.0, with 0.02 % Tween-20, 10 % Glycerol.内毒素
Less than 0.1 EU/ug of rHuFGF-16 as determined by LAL method.消融申明
贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.参考材料
Fibroblast growth factor 16 (FGF-16) belongs to the large FGF family. All FGF family members are heparin-binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-16 was originally identified in rat heart tissue by homology based polymerase chain reaction. Human FGF-16 cDNA predicts a 207 aa precursor protein with one N-linked glycosylation site. FGF-16 lacks a typical signal peptide, but is efficiently generated by mechanisms other than the classical protein secretion pathway. Among FGF family members, FGF-16 is most similar to FGF-9, sharing 73% aa sequence homology. Human FGF-16 shares 99% and 98.6% aa sequence identity with the mouse and rat FGF-16, respectively.纯度
> 98 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
