Recombinant Human Growth Regulated Protein-beta/CXCL2 (rHuGRO-beta/CXCL2)
英文全名
Recombinant Human Growth Regulated Protein-beta/CXCL2 (rHuGRO-beta/CXCL2)英文又名
MIP2-alpha卵白布局
氨基酸序列
APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSNAccession
Gene ID
产物英文概述
CXCL2, also named GRO-β, is belonging to the CXC chemokine family. It is encoded by the gene CXCL2 in humans. CXCL2/GRO-β shares 90 % amino acid sequence with CXCL1/GRO-alpha. All three human GROs (GRO-alpha, GRO-β, GRO-gamma) are members of the intercrine alpha (chemokine C-X-C) subfamily of chemokine. This chemokine is secreted by monocytes and macrophages. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. Similar to other GRO proteins, CXCL2 is potent neutrophil attractants and activators. In addition, it is also active toward basophils.来历
Escherichia coli.份子量
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.内毒素
Less than 1 EU/ug of rHuGRO-β/CXCL2 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
