Recombinant Human Hemofiltrate CC Chemokine-1/CCL14 (rHuHCC-1/CCL14)
英文全名
Recombinant Human Hemofiltrate CC Chemokine-1/CCL14 (rHuHCC-1/CCL14)英文又名
HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14卵白布局
氨基酸序列
TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK ENAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.内毒素
Less than 1 EU/ug of rHuHCC-1/CCL14 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Schulz-Knappe P, Magert HJ, Dewald B, et al. 1996. J Exp Med. 183:295-9.2. Detheux M, Standker L, Vakili J, et al. 2000. J Exp Med. 192:1501-8.3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13.纯度
> 96 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
