Recombinant Human Indian Hedgehog Cys28IleIle (rHuIHHC28II)
英文全名
Recombinant Human Indian Hedgehog Cys28IleIle (rHuIHHC28II)英文又名
HHG-2卵白布局
氨基酸序列
IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGGAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 ug/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4.内毒素
Less than 1 EU/ug of rHuIHH C28II as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Kumar S, Balczarek KA, Lai ZC. 1996. Genetics. 142:965-72.2. Marigo V, Roberts DJ, Lee SM, et al. 1995. Genomics. 28:44-51.3. Vortkamp A, Lee K, Lanske B, et al. 1996. Science. 273:613-22.纯度
> 96 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
